p pp2a c  (Santa Cruz Biotechnology)

Journal: Nutrition & Metabolism

Article Title: High fructose induces dysfunctional vasodilatation via PP2A-mediated eNOS Ser1177 dephosphorylation

doi: 10.1186/s12986-022-00659-3

Figure Lengend Snippet: High fructose affects the eNOS pathway closely related to PP2A in endothelial cells. MVECs were stimulated by fructose for 8 h. A, B Western blot analysis showing the protein expression of PP2AC and C, D P-PP2A (Tyr307) in MVECs. Relative protein levels of PP2AC and P-PP2A (Tyr307) were normalized to that of β-actin. Data are presented as the mean ± SEM (n = 3). C, control. * P < 0. 05, ** P < 0. 01 versus C

Article Snippet: Phosphate buffer solution powder (AR0030) and β-actin (1:1000, BM0627) antibodies were purchased from Boster Company, China; eNOS (1:1000, 32027), PP2AC (1:1000, 2038S), anti-mouse (3:5000, 4408S), anti-rabbit (1:1500,7076S) were purchased from CST, United States; P-PP2A (PP2ACα, Tyr 307; 1:1000, AF4453) and P-eNOS (Ser 1177; 1:1000, AF3247) were purchased from Affinity, China.

Techniques: Western Blot, Expressing, Control

Journal: Nutrition & Metabolism

Article Title: High fructose induces dysfunctional vasodilatation via PP2A-mediated eNOS Ser1177 dephosphorylation

doi: 10.1186/s12986-022-00659-3

Figure Lengend Snippet: PP2A inhibitor recovers high fructose-induced dysfunction mediated by NO reduction. MVECs were pretreated with or without 20 nM OA for 1 h and then stimulated with fructose. A NO production from MVECs (after pretreatment with fructose for 24 h) (n = 4). Western blot analysis showing the protein expression of eNOS, P-eNOS (Ser1177) ( B, C ), PP2AC, and P-PP2A (Tyr307) ( D, E ) in MVECs (after pretreatment with fructose for 8 h) (n = 3). Relative protein levels of P-eNOS (Ser1177) were normalized to that of eNOS, and those of P-PP2A (Tyr307) were normalized to that of PP2AC. All data are presented as the mean ± SEM. C, control; Fru, fructose. * P < 0.05, ** P < 0.01 when compared with the respective control groups

Article Snippet: Phosphate buffer solution powder (AR0030) and β-actin (1:1000, BM0627) antibodies were purchased from Boster Company, China; eNOS (1:1000, 32027), PP2AC (1:1000, 2038S), anti-mouse (3:5000, 4408S), anti-rabbit (1:1500,7076S) were purchased from CST, United States; P-PP2A (PP2ACα, Tyr 307; 1:1000, AF4453) and P-eNOS (Ser 1177; 1:1000, AF3247) were purchased from Affinity, China.

Techniques: Western Blot, Expressing, Control

Journal: Nutrition & Metabolism

Article Title: High fructose induces dysfunctional vasodilatation via PP2A-mediated eNOS Ser1177 dephosphorylation

doi: 10.1186/s12986-022-00659-3

Figure Lengend Snippet: Vasodilation function unchanged in the PP2A cKO mice under high fructose stimulation. A Identification of the PP2ACα cKO mice by genotyping. In each group of experiments, the aortic rings were stimulated by fructose (0.25 mM and 2 mM) for 0.5 h, precontracted by using 10 –6 M PE, and relaxed by 10 –9 –10 –5 M Ach or SNP. Wire myograph recording the effect of fructose in concentration–response curves to the endothelium-dependent dilator Ach ( B ) and the endothelium-independent dilator SNP ( C ) in aortic rings from the PP2ACα flox/flox and cKO mice. D, E The maximal response of Ach and SPN. All data are presented as the mean ± SEM (n = 5 aortic rings). C, control; Fru, fructose; Ach, acetylcholine; SNP, sodium nitroprusside; PE, phenylephrine. * P < 0. 05, ** P < 0. 01 when compared with the respective control groups

Article Snippet: Phosphate buffer solution powder (AR0030) and β-actin (1:1000, BM0627) antibodies were purchased from Boster Company, China; eNOS (1:1000, 32027), PP2AC (1:1000, 2038S), anti-mouse (3:5000, 4408S), anti-rabbit (1:1500,7076S) were purchased from CST, United States; P-PP2A (PP2ACα, Tyr 307; 1:1000, AF4453) and P-eNOS (Ser 1177; 1:1000, AF3247) were purchased from Affinity, China.

Techniques: Concentration Assay, Control